Team:Calgary/Project/DataPage
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<p><b><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902000">BBa_K902000</a></b> and <b><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902004">BBa_K902004,</a></b>: two novel hydrolase enzymes were submitted to the registry for the hydrolysis of two different sugar conjugated electroactive compounds. Used in conjunction with the existing lacZ part in the registry (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I732005">BBa_I732005,</a>), this allows for the electrochemical detection of three compounds with a single electrode. A uidA inducible generator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_k902002">BBa_k902002,</a>) was submitted and characterized electrochemically. This data can be found on our <a href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting">electroreporting page</a>. | <p><b><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902000">BBa_K902000</a></b> and <b><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902004">BBa_K902004,</a></b>: two novel hydrolase enzymes were submitted to the registry for the hydrolysis of two different sugar conjugated electroactive compounds. Used in conjunction with the existing lacZ part in the registry (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I732005">BBa_I732005,</a>), this allows for the electrochemical detection of three compounds with a single electrode. A uidA inducible generator (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_k902002">BBa_k902002,</a>) was submitted and characterized electrochemically. This data can be found on our <a href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting">electroreporting page</a>. | ||
- | <p><b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>,<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902023">BBa_K902023</a></b> and<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902074">BBa_K902074</a></b>: three novel riboswitchs were submtted along with two associated promoters (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) and <b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) and an inducible/repressible promoter <b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902065">BBa_K902065</a></b>) were submitted to the registry. One of these riboswitches (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) was tested with GFP (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902014">BBa_K902014</a></b>), with its promoter and GFP (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902017">BBa_K902017</a></b>) and with its promoter and the S7 kill gene (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902018">BBa_K902018</a></b>) This data can be found on our killswitch (<b><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">regulation</a></b>) page. | + | <p><b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>,<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902023">BBa_K902023</a></b> and<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902074">BBa_K902074</a></b>: three novel riboswitchs were submtted along with two associated promoters (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) and <b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) and an inducible/repressible promoter <b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902065">BBa_K902065</a></b>) were submitted to the registry. One of these riboswitches (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902008">BBa_K902008</a></b>) was tested with GFP using this construct, (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902014">BBa_K902014</a></b>), with its promoter and GFP using this construct (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902017">BBa_K902017</a></b>) and with its promoter and the S7 kill gene using this construct (<b><a href="http://partsregistry.org/wiki/index.php?title=BBa_K902018">BBa_K902018</a></b>) This data can be found on our killswitch (<b><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">regulation</a></b>) page. |
<a href="http://partsregistry.org/wiki/index.php?title=BBa_K902017">BBa_K902017</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902014">BBa_K902014</a> and <a href="http://partsregistry.org/wiki/index.php?title=BBa_K902018">BBa_K902018</a> - <b>BBa_</b> (Magnesium Dependent Promoter and Riboswitch GFP Generator) (Rhamnose Inducible Promoter GFP Generator) (MOCO Riboswitch GFP Generator) (Manganese Inducible Promoter Riboswitch GFP) </p> | <a href="http://partsregistry.org/wiki/index.php?title=BBa_K902017">BBa_K902017</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902014">BBa_K902014</a> and <a href="http://partsregistry.org/wiki/index.php?title=BBa_K902018">BBa_K902018</a> - <b>BBa_</b> (Magnesium Dependent Promoter and Riboswitch GFP Generator) (Rhamnose Inducible Promoter GFP Generator) (MOCO Riboswitch GFP Generator) (Manganese Inducible Promoter Riboswitch GFP) </p> |
Revision as of 19:51, 3 October 2012
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Detect and Destroy: Data Page
Design
Major Accomplishments
Data from New parts Submitted to the Registry
BBa_K902000 and BBa_K902004,: two novel hydrolase enzymes were submitted to the registry for the hydrolysis of two different sugar conjugated electroactive compounds. Used in conjunction with the existing lacZ part in the registry (BBa_I732005,), this allows for the electrochemical detection of three compounds with a single electrode. A uidA inducible generator (BBa_k902002,) was submitted and characterized electrochemically. This data can be found on our electroreporting page.
BBa_K902008,BBa_K902023 andBBa_K902074: three novel riboswitchs were submtted along with two associated promoters (BBa_K902008) and BBa_K902008) and an inducible/repressible promoter BBa_K902065) were submitted to the registry. One of these riboswitches (BBa_K902008) was tested with GFP using this construct, (BBa_K902014), with its promoter and GFP using this construct (BBa_K902017) and with its promoter and the S7 kill gene using this construct (BBa_K902018) This data can be found on our killswitch (regulation) page. BBa_K902017, BBa_K902014 and BBa_K902018 - BBa_ (Magnesium Dependent Promoter and Riboswitch GFP Generator) (Rhamnose Inducible Promoter GFP Generator) (MOCO Riboswitch GFP Generator) (Manganese Inducible Promoter Riboswitch GFP)
Kill Switch Generators - S7 constructs?!?!??!?!?!? also CviaII? What do we have ready to submit?
Decatecholization - Optimized an existing biobrick for the production of xylE (catechol dioxygenase) for the degradation of catechol using a constitutive promoter. Other OSCAR parts?!?!?!?
Parts Already In the Registry
Optimized the Petrobrick (BBa_590025) to be able to convert naphthenic acids into alkane and alkene compounds.
Additional Work and Characterizations
Developed a program using MATLAB for the optimization of metabolic pathways in synthetic biology metabolic networks. The program allows you to build an artificial synthetic biology network in E. coli and predicts substrates that should be fed to the organism to increase production of the compound. This was characterized and validated in the wetlab with the Petrobrick.
Developed hardware and software for the development of a biosensor using an electrochemical sensor. The software is available on our wiki. (Robert can you help me flush this out a bit, or one of the engineers?