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Team:Calgary/Notebook/Protocols/mgcircuit
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Revision as of 16:55, 3 October 2012
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Characterization of the magnesium circuit with GFP
- Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- mgtAp-rb-K082003
- mgtAp-rb
- R0040-mgtArb-K082003
- R0040-B0034-K082003
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl
- 0mM MgCl2
- 0.01mM MgCl2
- 0.1mM MgCl2
- 1mM MgCl2
- 10mM MgCl2
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.
