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Team:Calgary/Notebook/Protocols/mgcircuit
From 2012.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
<ul><li>mgtAp-rb-K082003</li> | <ul><li>mgtAp-rb-K082003</li> | ||
<li>mgtAp-rb</li> | <li>mgtAp-rb</li> | ||
| - | <li>R0040-mgtArb-K082003</li></ul> | + | <li>R0040-mgtArb-K082003</li> |
| - | <li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl<font style="text-transform: lowercase;">2</font>.</li> | + | <li>R0040-B0034-K082003</li></ul> |
| + | <li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl | ||
| + | <ul><li> 0mM MgCl<sub>2</sub></li> | ||
| + | <li> 0.01mM MgCl<sub>2</sub></li> | ||
| + | <li> 0.1mM MgCl<sub>2</sub></li> | ||
| + | <li> 1mM MgCl<sub>2</sub></li> | ||
| + | <li> 10mM MgCl<sub>2</sub></li><font style="text-transform: lowercase;">2</font>.</li> | ||
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li> | <li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li> | ||
</ol> | </ol> | ||
Revision as of 05:55, 3 October 2012
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Characterization of the magnesium circuit
- Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- mgtAp-rb-K082003
- mgtAp-rb
- R0040-mgtArb-K082003
- R0040-B0034-K082003
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl
- 0mM MgCl2
- 0.01mM MgCl2
- 0.1mM MgCl2
- 1mM MgCl2
- 10mM MgCl2 2.
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.
