Team:Valencia Biocampus/Project

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(Overall project)
(Who said scientists don't care about microbial cultures?)
 
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<h2>Overall project</h2>
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== '''Overall project''' ==
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Who said scientists don't care about microbial cultures?  
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== '''Who said scientists don't care about microbial cultures?''' ==
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This year, València Biocampus team members are going to break down communication frontiers among species through an artificial language based on wavelengths, which will allow us to know more about the requirements of the microorganisms we thrive everyday in our labs. Depending on different metabolic conditions, our microorganisms (bacteria and yeast) will express fluorescent proteins that are under the control of promoters sensitive to these nutrients. Therefore, by using a fluorimeter controlled by voice-recognition software, we are able to ask and get answers (like real-life talking!) of bacteria environment! Moreover we have a brand-new experience, related to UV-regulation, in which we dictate what the bacterium has to produce!
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This year, Valencia Biocampus team members are going to break down communication barriers among species through an artificial language based on light wavelengths, which will allow us to know more about the requirements of the microorganisms we thrive everyday in our labs. Depending on different metabolic conditions, our microorganisms (bacteria and yeast) will express fluorescent proteins that are under the control of promoters sensitive to these environmental factors. Therefore, by using a fluorimeter controlled by voice-recognition software, we are able to ask and get answers (like real-life talking!) of the microbial environment! Moreover we have a brand-new experience, related to UV-regulation, in which we force bacteria to express a gene!<br>
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Our final goal is to characterize and  completely model bacterial behavior affected by these factors. And last, but not least, our Human Practices team has an awesome story-telling script for the audience!<br><br>
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We have achieved good results so far. After ordering a total number of 7 different constructions (5 for bacteria, 2 for yeast) synthesized chemically we transformed competent cells and they responded very well to their medium conditions. We are, right now, working on improving the nutrient-sensing part.
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This is a general scheme of our project. All the experimental work is outlined and splitted up into color boxes: <html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Modeling"><b>Modeling</b></a></html> (purple), <html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Ethics"><b>Human Practices</b></a></html> (blue) and <html><a href="https://2012.igem.org/Team:Valencia_Biocampus/talking"><b>Talking Interfaces</b></a></html> (yellow). As long as you surf through the wiki, you will find out the special case of the question <html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Ethics#Talking_Life"><b>“Have you taken your pill?”</b></a></html>, which has not been actually asked to our bacteria, but plays a central role in our Human Practices work. Check our <html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Achievements"><b>achievements</b></a></html> to understand our project as a whole!
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Our final goal is to characterize and model completely well bacteria interaction regarding to these nutrient factors, how they behave then and how they answer to the different conditions. In addition, we would like to design a self-made-but-cool device for carrying out the whole trial. And last, but not least, our Human Practices team has an awesome story-telling script for the audience!
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We are looking forward to seeing you all and enjoy together this amazing experience! ☺
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        <td>[[Image:Bacterium_vlcampus.png|100px]]</td>
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        <td>Resumen bacterias</td>
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        <td>Our aim in this part of the project is to detect when the yeast starts fermenting. At the end of the project we will be able to “ask” the yeast if there is still any glucose in the medium or not through the addition of H2O2. Furthermore, we will be able to know for how long the media has been running out of glucose. In conclusion, this project allows us to know how much time has elapsed since the fermentation began.</td>
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== Project Details==
== Project Details==
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== Results ==
== Results ==
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Latest revision as of 20:46, 26 September 2012




Overall project


Who said scientists don't care about microbial cultures?

This year, Valencia Biocampus team members are going to break down communication barriers among species through an artificial language based on light wavelengths, which will allow us to know more about the requirements of the microorganisms we thrive everyday in our labs. Depending on different metabolic conditions, our microorganisms (bacteria and yeast) will express fluorescent proteins that are under the control of promoters sensitive to these environmental factors. Therefore, by using a fluorimeter controlled by voice-recognition software, we are able to ask and get answers (like real-life talking!) of the microbial environment! Moreover we have a brand-new experience, related to UV-regulation, in which we force bacteria to express a gene!
Our final goal is to characterize and completely model bacterial behavior affected by these factors. And last, but not least, our Human Practices team has an awesome story-telling script for the audience!

This is a general scheme of our project. All the experimental work is outlined and splitted up into color boxes: Modeling (purple), Human Practices (blue) and Talking Interfaces (yellow). As long as you surf through the wiki, you will find out the special case of the question “Have you taken your pill?”, which has not been actually asked to our bacteria, but plays a central role in our Human Practices work. Check our achievements to understand our project as a whole!



Overview scheme vlc3.png