Team:University College London/Notebook/Week14

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== Tuesday 11th September ==
== Tuesday 11th September ==
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<div class="notebook-meeting"> '''Darmstadt Collaboration''' Bouran swapped address with Henrik from Darmstadt today, we'll be sending them Laccase for characetrisation, while they are sending us a cutinase enzyme for expression in our Roseobacter.</div>
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<div class="notebook-meeting"> '''Darmstadt Collaboration''' </div>
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<div class="notebook-meeting"> '''Purdue Collaboration''' James was in touch with Chris from Purdue again to discuss swapping protocols for curli characteristion. </div>
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<div class="notebook-meeting"> '''Sponsume''' The crowdfundraising website posted a short article Philipp wrote about science fundraising for short-term projects like iGEM: http://www.sponsume.com/blog/university-college-london-igems-experiment-crowdfunding </div>
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<div class="notebook-meeting"> '''Lab clean-up''' Safety always first. We had a huge clean-up of the lab, including scrubbing the microwave spotless! We're now ready for our last month in the lab. </div>
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<div class="notebook-meeting"> '''Lab clean-up''' </div>
== Wednesday 12th September ==
== Wednesday 12th September ==
<div class="notebook-meeting"> '''Practice Presentation''' Today the team presented to our supervisors and received some good feedback. Overall we really need to improve the clarity of our presentation, it's hard to concisely summarise a summer of work.</div>
<div class="notebook-meeting"> '''Practice Presentation''' Today the team presented to our supervisors and received some good feedback. Overall we really need to improve the clarity of our presentation, it's hard to concisely summarise a summer of work.</div>
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<div class="notebook-meeting"> '''Hackspace Day 1''' We went en masse to the London Hackspace this evening for the first workshop. Carina, Yeping, Philipp, Martina and Bethan were all present. Howard met us there. We bought a glycerol stock of W3110 ''E. Coli'' and empty petri dishes to show the biohackers how we make minimal agar plates for day 1 protocol of making competent cells. Quite a few people turned up and they were very keen to have a go!</div>
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<div class="notebook-meeting"> ''iGEM connection''' Bethan is organising with iGEM Paris-Bettencourt a trip to Paris to discuss how our two project approach biosafety, and also discuss aspects of our DIYbio collaboration with members of the CRI's Education X.0 project. Paris iGEM also invited us to participate in their debate on the 16th! </div>
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<div class="notebook-meeting"> '''Hackspace Day 1''' </div>
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<div class="notebook-meeting"> ''iGEM connection''' </div>
== Thursday 13th September ==
== Thursday 13th September ==

Revision as of 20:58, 25 September 2012

Contents

Notebook: Week 14

Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16


Aims of the Week

Monday 10th September

Paris visit
Hackspace Yeping finished planning the workshops for the collaboration with the London Hackspace. We're really looking forward to the workshops starting on Wednesday!

Tuesday 11th September

Darmstadt Collaboration
Sponsume The crowdfundraising website posted a short article Philipp wrote about science fundraising for short-term projects like iGEM: http://www.sponsume.com/blog/university-college-london-igems-experiment-crowdfunding
Lab clean-up

Wednesday 12th September

Practice Presentation Today the team presented to our supervisors and received some good feedback. Overall we really need to improve the clarity of our presentation, it's hard to concisely summarise a summer of work.
Hackspace Day 1
iGEM connection'

Thursday 13th September

Hackspace Day 2 Together with the hackers, Bethan helped to build a shaker to agitate our cells overnight. This involved using a number of equipment only available at the Hackspace, including hand saw, glue gun and motor. The biohackers were very keen to give us an electronics tutorial as we build our circuit for the shaker. A variable resistor was included in the circuit design so we can control the speed of the shaker.
We also performed PCR of the cholorophenicol plasmid backbone required for our ligations. At hackspace, they have a very cool 'ancient' thermocycler. It has no heated lid so mineral oil has to be added to samples to prevent evaporation. The biohackers showed us how to program the machine. There are so many steps compared to the one used in our lab!

Friday 14th September

Hackspace Day 3 Our shaker from yesterday was still alive today and the liquid cultures in falcon tubes are all cloudy showing growth! Together with the biohackers we ran a 1% gel of the PCR product from yesterday using their DIY gel tray. Their tray didn't have a cover, you can also see how the wires are connected! Using a hand held UV filter and UV light, we visualized our gel. The PCR has worked! Gel shows the expected band at 2070bp. PCR clean-up is to be done at the UCL workshops since the centrifuge at Hackspace is only estimated to be able to do 5000rpm
Press C-lab published an exciting write-up of our 1st day at the Hackspace


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