Team:Valencia Biocampus/Bacterium

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<h2>Bacteria Subteam</h2>
<h2>Bacteria Subteam</h2>
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=== '''THE IDEA''' ===
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We have developed a simple and fast method of determining "the comfort" of the bacterial culture by asking them four questions related with their metabolic state which are replied in the form of voice answers. Moreover, we have engineered another genetic construction to order them to produce a protein.
Here is an overview of how our bacteria work. For more information look the '''molecular mechanisms''' below.
Here is an overview of how our bacteria work. For more information look the '''molecular mechanisms''' below.
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==='''BACTERIAL SYNTHAXIS'''===
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This is the Rosseta stone we used to define both human and bacteria language:
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[[Image:Tabla_synthaxis.png|700 px||center]]
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<br><br>
==='''MOLECULAR MECHANISMS'''===
==='''MOLECULAR MECHANISMS'''===
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Click on each plasmid to learn more about how our constructions work!
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<table align="center" border="0.01" bordercolor="#9F9F9F" style="background-color:#9F9F9F">
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        <td><html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Molecular#LACTOSE-INDUCED_PROMOTER">
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        <img src="https://static.igem.org/mediawiki/2012/6/66/Bacteria_hungry.png" width="230" height="200"</a></html></td>
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        <td><html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Molecular#NITROGEN-REGULATED_PROMOTER">
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        <img src="https://static.igem.org/mediawiki/2012/7/76/Bacteria_nitrogen.png" width="230" height="200"</a></html></td>
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        <td><html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Molecular#OXYGEN-REGULATED_PROMOTER">
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        <img src="https://static.igem.org/mediawiki/2012/f/f4/Bacteria_oxygen.png" width="230" height="200"</a></html></td>
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        <td><html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Molecular#TEMPERATURE-INDUCED_PROMOTER">
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        <img src="https://static.igem.org/mediawiki/2012/3/38/Bacteria_hot.png" width="230" height="200"</a></html></td>
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        <td><html><a href="https://2012.igem.org/Team:Valencia_Biocampus/Molecular#UV-INDUCED_PROMOTER">
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        <img src="https://static.igem.org/mediawiki/2012/3/3d/Bacteria_expressgene.png" width="230" height="200"</a></html></td>
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        <img src="https://static.igem.org/mediawiki/2012/0/0f/Fondo_gris.png" width="230" height="200"</a></html></td>
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'''LACTOSE-INDUCED PROMOTER'''
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=== '''EXPERIMENTAL OUTLINE''' ===
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This construction is made up of three parts:
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Here you have a graphical overview of the experimental approach we followed:
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    <li> The transcription factor-binding site insidethe promoter,
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    <li> the repressor-binding site outside the promoter and
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    <li> the coding sequence, which contains a synthetic fluorescent (blue) protein. Our construction uses the well-known lactose operon system (1).
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        Since there is an operator region that blocks transcription, it is necessary to know it and avoid it.
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[[Image:Outline_ppt.png|700 px||center]]

Latest revision as of 19:02, 26 September 2012



    Bacteria Subteam


    THE IDEA


    We have developed a simple and fast method of determining "the comfort" of the bacterial culture by asking them four questions related with their metabolic state which are replied in the form of voice answers. Moreover, we have engineered another genetic construction to order them to produce a protein. Here is an overview of how our bacteria work. For more information look the molecular mechanisms below.



    BACTERIAL SYNTHAXIS


    This is the Rosseta stone we used to define both human and bacteria language:

    Tabla synthaxis.png



    MOLECULAR MECHANISMS


    Click on each plasmid to learn more about how our constructions work!



    EXPERIMENTAL OUTLINE


    Here you have a graphical overview of the experimental approach we followed:

    Outline ppt.png
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