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Team:University College London/LabBook/Week15
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| + | <FONT SIZE=+1>Moday (17.09.12) 3A assembly</FONT SIZE=+1> <p> | ||
| + | <b>Aim – Start 3A assembly to ligate: </b></p> | ||
| + | - the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> | ||
| + | - the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct</p> | ||
| + | - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> | ||
| + | - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct</p> | ||
| + | - pstA promoter (BBA_I719005) and the GFP (BBA_I712069)</p> | ||
| + | - the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.</p> | ||
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== 15.2 == | == 15.2 == | ||
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Revision as of 16:25, 26 September 2012
15.1
Moday (17.09.12) 3A assembly
Aim – Start 3A assembly to ligate:
- the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct - the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct - the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct - pstA promoter (BBA_I719005) and the GFP (BBA_I712069) - the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT.15.2
