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Team:Groningen/Notebook/Wetwork 5August2012
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Nisa / Emeraldo<br><br> | Nisa / Emeraldo<br><br> | ||
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6.) pSac-CM + lycopene<br> | 6.) pSac-CM + lycopene<br> | ||
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)<br> | 7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)<br> | ||
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| - | PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS. | + | PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.<br> |
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| + | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Revision as of 13:14, 26 September 2012
Nisa / Emeraldo
Plasmid isolation from E. coli:
1.) 4 colonies alsT (150) promoter + GFP in pSB1C3
2.) 4 colonies alsT (250) promoter + GFP in pSB1C3
3.) 4 colonies alsT (300) promoter + GFP in pSB1C3
4.) 4 colonies alsT (500) promoter + GFP in pSB1C3
5.) 2 colonies of LacI promoter in pSB1C3
6.) pSac-CM + lycopene
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.
Back to notebook
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