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From 2012.igem.org

Nisa / Emeraldo

Plasmid isolation from E. coli:
1.) 4 colonies alsT (150) promoter + GFP in pSB1C3
2.) 4 colonies alsT (250) promoter + GFP in pSB1C3
3.) 4 colonies alsT (300) promoter + GFP in pSB1C3
4.) 4 colonies alsT (500) promoter + GFP in pSB1C3
5.) 2 colonies of LacI promoter in pSB1C3
6.) pSac-CM + lycopene
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)

PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.


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