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Team:Groningen/Notebook/Wetwork 26June2012
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Colonies containing LuxI and LuxR were incubated in LB overnight. | Colonies containing LuxI and LuxR were incubated in LB overnight. | ||
| + | |||
| + | Nisa and Emeraldo | ||
| + | 1.)Plasmid isolation from O/N E. coli with BBa_K090403 gave a low yield (<30ng/ul) | ||
| + | 2.)Restriction of BBa_K090403 and the pigments with EcoRI and SpeI failed, no multiple bands detected on gel | ||
| + | 3.)Plan for single enzyme restriction. | ||
| + | 4.)The B. subtilis transformation is postponed | ||
Revision as of 16:55, 8 July 2012
Tom
Plasmid issolation of BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)).
Concentrations were low: LuxR (1) = 12.7 ng/ul, LuxR (2) = 25.2 ng/ul, LuxI (1) = 24.8 ng/ul, LuxI (2) = 22.3 ng/ul.
Colonies containing LuxI and LuxR were incubated in LB overnight.
Nisa and Emeraldo 1.)Plasmid isolation from O/N E. coli with BBa_K090403 gave a low yield (<30ng/ul) 2.)Restriction of BBa_K090403 and the pigments with EcoRI and SpeI failed, no multiple bands detected on gel 3.)Plan for single enzyme restriction. 4.)The B. subtilis transformation is postponed
