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Team:Groningen/Notebook/Wetwork 21August2012
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| + | Tom<br> | ||
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| + | Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius. <br> | ||
| + | <br> | ||
| + | Result: None of the combined promotors with GFP resulted in the correct size band.<br> | ||
| + | <br> | ||
Emeraldo<br> | Emeraldo<br> | ||
| - | Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product. | + | Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product.<br> |
<br><br> | <br><br> | ||
Arjan | Arjan | ||
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GC-MS: blank runs of the three organic solvents used<br> | GC-MS: blank runs of the three organic solvents used<br> | ||
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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{{Template:SponsorsGroningen2012}} | {{Template:SponsorsGroningen2012}} | ||
Revision as of 13:42, 26 September 2012
Tom
Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees celcius.
Result: None of the combined promotors with GFP resulted in the correct size band.
Emeraldo
Cut AmilGFP, BBa_K274110, and pSac-Cm+terminator with EcoRI and PstI, run on the gel and excised the correct bands (the AmilGFP band was not detected). Ligation reaction for cut BBa_k274110 and cut pSac-CM+terminator. Transformation of E.coli DH5alpha with the ligated product.
Arjan
GC-MS: blank runs of the three organic solvents used
Back to notebook
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