Team:Grenoble/Project/Main Results
From 2012.igem.org
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- | This part will present the main results we obtained pre-regional jamboree. | + | This part will present the main results we obtained pre-regional jamboree.<br/> |
- | In a first place, biologists and modellers worked on a simple amplification loop. | + | In a first place, biologists and modellers worked on a simple amplification loop.<br/> |
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. (Figure 1). | The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. (Figure 1). | ||
+ | </section> | ||
+ | <section> | ||
The team thought that an AND gate could be our solution. It could add a biological noise filter and resolve the problem of the continuous expression of GFP. | The team thought that an AND gate could be our solution. It could add a biological noise filter and resolve the problem of the continuous expression of GFP. | ||
Modelling results confirmed that built system will work. (Figure2) | Modelling results confirmed that built system will work. (Figure2) |
Revision as of 23:17, 26 September 2012
Main results
In a first place, biologists and modellers worked on a simple amplification loop.
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed. (Figure 1).
This can be attributed to the fact that overproduction of cAMP is lethal to Escherichia coli possibly due to an accumulation of methylglyoxal [http://jb.asm.org/content/119/2/357.abstract] [http://mic.sgmjournals.org/content/151/3/707.abstract].
In the future weeks (according to ours results) we will try to make the culture of our transformed bacteria in a medium complement with glucose and acetate. A possible track would be to add another regulation system on the adenylate cyclase production with the riboswitch RsmA/rsmY.