Team:Grenoble/Biology/Protocols

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iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
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Project

Protocols

Here you can find the protocols used by the team. There are

Cell culture

  • Medium compositions
  • LB Agar plates preparation
  • Glycerol stock production

BioBricks utilisation

  • BioBricks extraction
  • Chemically competent cells production
  • Transformation of E. coli competent cells (protocol 1)
  • Transformation of E. coli competent cells (protocol 2)

Molecular biological techniques

  • PCR using a GoTaq polymerase
  • PCR using a HF Phusion polymerase
  • Gibson Assembly
  • Restriction enzyme digest
  • Ligation
  • Miniprep

Gel migration

  • Gel electrophoresis
  • DNA extraction

Test techniques

  • "AND" gate test

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