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Team:Grenoble/Biology/Protocols
From 2012.igem.org
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| - | <li>"AND" gate test</li> | + | <li>"AND" gate test (control of pBAD expression by cAMP and arabinose)</li> |
</ul> | </ul> | ||
</section> | </section> | ||
Revision as of 13:20, 22 August 2012
Protocols
Here you can find the protocols used by the team. There areCell culture
- Medium compositions
- LB Agar plates preparation
- Glycerol stock production
BioBricks utilisation
- BioBricks extraction
- Chemically competent cells production
- Transformation of E. coli competent cells (protocol 1)
- Transformation of E. coli competent cells (protocol 2)
Molecular biological techniques
- PCR using a GoTaq polymerase
- PCR using a HF Phusion polymerase
- Gibson Assembly
- Restriction enzyme digest
- Ligation
- Miniprep
Gel migration
- Gel electrophoresis
- DNA extraction
Test techniques
- "AND" gate test (control of pBAD expression by cAMP and arabinose)
