Team:Grenoble/Biology/Notebook/September

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Revision as of 02:11, 27 September 2012

iGEM Grenoble 2012

Project

August

Week 31Week 32Week 33Week 34Week 35

Week 36: September 03rd to September 09th

Goal of the week:

We tested the double mutant strains construction (protocol).
We did some verification colony PCRs with HF Phusion enzyme (protocol) in order to check the constructions.

To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_28

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

  • Lane 1:

photo_gel_29

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)