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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | <p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | ||
<ul> | <ul> | ||
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<h2>General Protocols</h2> | <h2>General Protocols</h2> | ||
| - | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li> | |
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/ | + | |
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | ||
| - | |||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/ | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Overnight Cultures</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Overnight Cultures</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/pcrpurification">PCR Purification</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/plasmidminiprep">Plasmid Purification (from <i>E. coli</i>)</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/compcells">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/compcells">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/taqpcr">Taq PCR Protocol</a></li> | ||
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| + | <h2>Electrochemistry Protocols</h2> | ||
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</ul> | </ul> | ||
Revision as of 01:50, 2 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Taq PCR Protocol
Electrochemistry Protocols
