LB Agar plates preparation

Goal

Prepare solid medium to culture bacteria.

Prepare the LB Agar

In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium. Then sterilize it using the autoclave.
You can store it at room temperature, but before use it, you need to heat it up.

Add the right antibiotic

If you want to prepare LB Agar plates added with antibiotics you need to:

heat the LB Agar up, in order to solubilize it
after the solubilization, wait for the solution to refresh
when the solution is not so hot (around 50°C), add the antibiotic solution (in a flow hood of course)

Antibiotics concentrations:

Antibiotics	Finale concentration
Chloramphenicol
Kanamycin
Ampicillin

Pour the plates

When the antibiotic solution is added or when the bottle is not so hot (around 50°C), you can pour the plates:

In a flow hood, pour approximately 15mL of the LB Agar solution into every plates
Let each plate cool until its solid
When it's ok, flip the plates, write on the back and store them at 4°C

Team:Grenoble/Biology/Protocols/LB

From 2012.igem.org