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Team:Grenoble/Biology/Notebook/June/week 26
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Week 26: June 25th to July 01st
For the detection module, we decided to work on a modified membrane receptor. It consists of two merged E. coli membrane receptors. The extracellular part is the extracellular part of Tap, a dipeptide membrane receptor. The cytoplasmic part is the cytoplasmic part of the EnvZ receptor, which has the ability to activate OmpR (by phosphorilation) which is a transcription factor.
It will be a proof of concept for a bigger system which is a complete pathogene detection module :
1st network : with amplifier 1 (RsmA-rsmY)
Amplifier 1 : RsmA-rsmY system characterisation: (3 final plasmids)
Plasmid Mapping
On a pSB4K5 plasmid, we wanted to put the construction: pLAC_fha1_eCFPBiobricks involved
pLAC_RBS (BBa_I13601) => final length ≃ 90bppLAC (BBa_I13601) => final length ≃ 90bp
eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp
plasmid pSB4K5 => final length ≃ 2400bp
plasmid pSB3C5 => final length ≃ 2400bp
plasmid pSB1A3 => final length ≃ 2400bp
New parts
RsmA (iGEM Grenoble 2011) => final length ≃ 200bprsmY (iGEM Grenoble 2011) => final length ≃ 170bp
fha1 (iGEM Grenoble 2011) => final length ≃ 80bp
