Team:Calgary/Notebook/Protocols/m9media

From 2012.igem.org

Revision as of 00:46, 4 October 2012 by Yqi2011 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!

M9 Minimal Media Preparation

  1. Dissolve the following salts in about 500mL of ddH2O:
    • 12.8 grams anhydrous Na2HPO4
    • 3.0 grams KH2PO4
    • 0.5 grams NaCl
    • 1.0 gram NH4Cl
  2. Bring the media to approximately 950 ml with ddH2O and pH to 7.4 with NaOH.
  3. Autoclave for 20 minutes on slow exhaust. Store at 4°C when finished.
  4. The following salts were dissolved in 50mL of water and cold filtered with a sterilized 0.22 micron filter:
    • 10.8 grams glucose
    • 0.19g of MgCl2
    • 0.0152 grams CaCl2.2H2O
    • 0.0100 grams Thiamine
    • 0.007g of FeCl2.4H2O
  5. Add the filter sterilized solution into the autoclaved media along with any other additives (e.g. antibiotics). Store at 4°C.



Retrieved from "http://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media"