Revival of Freeze-dried Bacterial Cultures from DMSZ

1. Remove the glass ampoule from plastic tube.
2. Check indicator to ensure intact seal (red or blue – good, pink or orange – humidity in the sample).
3. Heat tip (pointy tip up) of glass ampoule over flame thoroughly.
4. Drop water onto tip to crack glass all around.
5. Carefully strike glass with forceps or similar tool to break off tip.
6. Remove insulation material and extract smaller vial with forceps. If the bacteria in the vial are anaerobic – ensure anaerobic conditions are followed during the rest of the culture revival.
7. Remove cotton plug from vial and keep it sterile.
8. Add 0.5 mL recommended medium to the dehydrated culture and replace cotton plug. Let the culture rehydrate for about 30 min.
9. Remove cotton plug again. Mix very gently with something like an inoculation loop and transfer to about 5mL liquid medium in a tube.
10. Drop 100uL of liquid culture onto plate and streak.
11. Incubation directions on DSMZ website.