Team:Calgary/Notebook/Protocols/catalase

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Catalase Assay

  1. Grow cultures of E. coli J04500-katG+LAA and J04500 overnight at 37*C in LB with appropriate antibiotics.
  2. The following evening, innoculate 20 uL into 3 mL LB with varying concentrations of hydrogen peroxide (0 mM, 1 mM, 5 mM, 10 mM) and grow overnight.
  3. The following morning, observe and record culture turbidity.














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