Protocols

Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.

Microalgae Protocols

• Preparation of Algal f2 Media
• Glass Bead Algal Transformation
• Chloroplast Isolation
• Nuclear DNA Extraction from Algae

Pseudomonas Biotinylation Techniques

• Plasmid Isolation (from Pseudomonas spp.)
• Genomic DNA Isolation (from Pseudomonas spp.)
• Biotinylation/Immunoprecipitation of Naphthenic Acids
• Conjugation of E. coli plasmids to Pseudomonas spp.

Electrochemical Techniques

• Electrode Plating
• Buffer Preparation
• Electrical Settings
• Testing For Oxidation

General Protocols

• Plasmid Isolation (from E. coli)
• RNA Extraction
• cDNA Synthesis
• Quantitative Real Time PCR
• Testing the pBAD-lacZ Construct
• Taq PCR Protocol
• PCR Purification
• Gel Extraction
• Testing the lacI-lacZ I732901 Gene
• Bacterial Transformation
• Rehydration of Registry DNA
• Construction Techniques
• Agarose Gel Electrophresis
• LB Agar Plates
• Overnight Cultures
• Preparing Chemically Competent Cells (E. coli)
• Preparing Glycerol Stocks (E. coli)