Team:Grenoble/Biology/Notebook/September

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iGEM Grenoble 2012

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August

Week 31 • Week 32 • Week 33 • Week 34 • Week 35

Week 36: September 03rd to September 09th

Goal of the week:

We tested the double mutant strains construction (protocol).
We did some verification colony PCRs with HF Phusion enzyme (protocol) in order to check the constructions.

To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_28

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

  • Lane 1:

photo_gel_29

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

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