Team:Valencia/protocols

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Culture medium

• LB liquid
• LB Agar Plates
• BG-11 for Wildtype Liquid
• BG-11 for Wildtype Solid
• BG-11 for Harvard/Golden Liquid
• BG-11 for Harvard/Golden Solid
• Compo Plus liquid
• Compo Plus Agar

Culture

• Culturing E.Coli
• Culturing S. elongatus in liquid medium
• Culturing S. elongatus in solid medium

Molecular Tools

• Transforming Chemically Competent Cells E.Coli DH5α-T1^R
• Plasmid Miniprep
• DNA Digestion
• Ligations
• Agarose Gel Electrophoresis
• Transforming: S. elongatus PCC7942