Team:University College London/LabBook/Week15

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Revision as of 17:53, 26 September 2012 by Zcbths3 (Talk | contribs)

15.1

Moday (17.09.12) 3A assembly


Aim – Start 3A assembly to ligate:

  • the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct
  • the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct
  • the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct
  • the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct
  • pstA promoter (BBA_I719005) and the GFP (BBA_I712069)

- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT. </p>

15.2

15.3

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