"

From 2012.igem.org

Renske

Measured FNR-GFP inside psac-cm inside B.subtilis 168. Same setup as 12/09.

After setup OD =

• 0.889 (rotten meat, FNR-GFP)
• 1.040 (no meat, WT)
• 0.675 (no meat, FNR-GFP)
• 1.435 (no meat, FNR-GFP, not inoculated again (old culture to see wether stationary phase induces GFP))

further microscopic part is executed by Arjan/Elbrich: no results.

Elbrich

pSac-Cm + RFP NanoDrop

1: 231,3 ng/ul
2: 131,0 ng/ul
3: 238,9 ng/ul
4: 166,8 ng/ul

After cutting, sizes on gel were correct: 2 bands, one at 5000 bp (pSac-Cm) and one at 1000 bp (RFP)


Back to notebook