Team:Grenoble/Biology/Protocols/Gel
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Gel electrophoresis
Goal
Separate DNA strands of different lengths.Prepare the gel
- Add 1 g of agarose powder to 50 mL of 1X TAE buffer.
- Heat the solution using the microwave until the powder is completely dissolved.
- Once the solution is cool enough to be touched, pour it into gel cast (don't forget the comb).
Load the gel
- When the gel is solid, remove carefully the comb.
- Place the gel in the buffer tank and cover it with the buffer solution.
- Load each sample into the wells (don't forget the loading dye and the DNA ladder).