Team:Grenoble/Biology/Protocols/Glycerol
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Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- Grow a liquid culture overnight.
- Prepare sterile 80% glycerol.
- Mix 200 µL of glycerol and 800 µL of liquid culture.
Then store the glycerol stock at -80°C.