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From 2012.igem.org

Tom

Plasmid issolation of BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)).

Concentrations were low: LuxR (1) = 12.7 ng/ul, LuxR (2) = 25.2 ng/ul, LuxI (1) = 24.8 ng/ul, LuxI (2) = 22.3 ng/ul.

Colonies containing LuxI and LuxR were incubated in LB overnight.

Nisa and Emeraldo 1.)Plasmid isolation from O/N E. coli with BBa_K090403 gave a low yield (<30ng/ul) 2.)Restriction of BBa_K090403 and the pigments with EcoRI and SpeI failed, no multiple bands detected on gel 3.)Plan for single enzyme restriction. 4.)The B. subtilis transformation is postponed