Escaper assay for the rhamnose system

The following assay is based on CFU in both Top 10 and glyA knockout of E. coli.

• The glyA auxotroph was grown in LB media overnight at 37^oC with shaking.
• Cells were washed in M9 minimal media two times and spun in between at 4,000 RPM at 4^oC for 10 min. each time.
• Finally, washed cells were subcultured into 5 mL of M9 minimal media supplemented with 1% (w/v) glucose and 50 ug/mL Kanamycin.
• Cells were grown at 37^oC with shaking for 12 and 24 hours and OD₆₀₀ measurements were used to quantitate growth.