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Team:Calgary/Notebook/Protocols/nucleaseassay
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Nuclease assay
- E. coli genome was prepared using MP bio kit and the DNA was quantified using picogreen assay.
- 2µg of DNA was put into each test condition.
- Appropriate buffer(s) were added for each enzyme/enzyme combination in the tubes.
- 10 units of enzyme was added into appropriate conditions
- At each timepoint aliquots were taken out of the reaction tubes and run on a 1% gel for 40 minutes and imaged.
