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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Purification (from E. coli)
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
