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Team:Grenoble/Project/Abstract
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sEnsiColi: A tunable and reliable ultra-sensitive detector
Multi-resistant bacteria are a worldwide issue which in a very near future will have huge impacts on our societies and ways diagnosis and prevention will be performed. In this optic, the Grenoble iGEM team has built an ultra-sensitive pathogen detector. It consists of three interconnected modules: 1- Detection, 2- Amplification/Communication and 3- Output. The detection module consists of a recombinant membrane receptor that, once activated, actuates an amplification loop. The amplification system contains a genetic feed forward loop, which filters out false positive outputs. Once amplified and filtered, the signal is transmitted to neighboring bacteria via a diffusible molecule. In turn, the amplification loop is triggered which leads to the production of a measurable fluorescence output. The design of our network is easily adaptable to different input signals by using other receptor domains.Scheme of the concept
Theoretical response of the device depending on the input signal
