Week 23 : Choice and description of the project.
Week 24 : RsmA-rsmY system (amplifier) design and research on a possible detection module.
Week 25 : Second amplifier design (cAMP-CRP system).
Week 26 : Detection module description, RsmA-rsmY system and cAMP-CRP network. Planning of first experiments.
July
Week 27 : Test PCR conditions, recovering and amplifying the biobricks involved in our genetic networks --> no significant results.
Week 28 : Recovery and amplification of the different biobricks --> Amplification of pLAC (fha1) // pLAC (rsmY) // pLAC_RBS // RsmA // rsmY // pSB1A3 // pSB3C5 (Cya) // pSB3C5 (RsmA).
Week 29 : Gibson assembly, transformation, amplification and recovery of biobricks --> successful Gibson Assembly: pLAC_RBS_RsmA on pSB3C5.
Week 30 : Amplification of pAra/Bad_RBS_GFP // RBS_Cya // fha // pSB4C5.
"
