Team:Grenoble/Biology/Protocols/Glycerol

From 2012.igem.org

Revision as of 09:53, 24 August 2012 by Adeline millet (Talk | contribs)

iGEM Grenoble 2012

Project

Glycerol stock preparation

Goal

Prepare glycerol stock is a way to store bacteria during a long period.

Protocol

You need to:
  1. grow a liquid culture overnight
  2. prepare sterile 80% glycerol
  3. mix 200 µL of glycerol and 800 µL of liquid culture

Then store the glycerol stock at -80°C.