Team:Grenoble/Biology/Protocols/Glycerol
From 2012.igem.org
Revision as of 09:53, 24 August 2012 by Adeline millet (Talk | contribs)
Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- grow a liquid culture overnight
- prepare sterile 80% glycerol
- mix 200 µL of glycerol and 800 µL of liquid culture
Then store the glycerol stock at -80°C.