Team:Grenoble/Biology/Protocols/Glycerol

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iGEM Grenoble 2012

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Glycerol stock preparation

Goal

Prepare glycerol stock is a way to store bacteria during a long period.

Protocol

You need to:

grow a liquid culture overnight
prepare sterile 80% glycerol
mix 200 L of glycerol and 800 L of liquid culture

Then store the glycerol stock at -80°C.

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