User contributions
From 2012.igem.org
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- 23:22, 3 October 2012 (diff | hist) N User:DrJones1935/18 July 2012 (Created page with "====I. Agarose Gel Electrophoresis (AGE) of PCR Samples==== *'''Make Gel:''' :*Measure out <strike>0.5 g</strike> 0.50497 g of agarose and add to a 250 mL E-flask :*Add 50 mL o...") (top)
- 23:22, 3 October 2012 (diff | hist) N User:DrJones1935/17 July 2012 (Created page with "====I. Dilute primers to 10 uM working stock==== *Mix: :*2 uL 200 uM primer stock :*38 uL sterile, DNAse-, RNAse-free dH<sub>2</sub>O *''Note: I think that the p5 primers got mi...") (top)
- 23:21, 3 October 2012 (diff | hist) N User:DrJones1935/16 July 2012 (Created page with "====I. Ordered Cross-over PCR Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm (Tm overhang/Tm ann...")
- 23:21, 3 October 2012 (diff | hist) N User:DrJones1935/15 July 2012 (Created page with "====I. Check Cultures for Growth==== ''RESULTS (~09:30)'': *Overnight cultures of plasmid strain pBAV1K-T5-gfp both grew robustly overnight. I will not centrifuge them together ...") (top)
- 23:19, 3 October 2012 (diff | hist) N User:DrJones1935/13 July 2012 (Created page with "====I. Check Plates for Growth==== *''RESULTS'': All overnight plates of ''B. subtilis'' showed growth, including the plates from two days ago left on the bench top. I have saved...") (top)
- 23:18, 3 October 2012 (diff | hist) User:DrJones1935/12 July 2012 (→I: Check WT B. subtilis Liquid Culture for Growth) (top)
- 23:17, 3 October 2012 (diff | hist) N User:DrJones1935/11 July 2012 (Created page with "====I. Check Plates and Cultures for Growth==== ''RESULTS (~09:45)'': *All plates showed growth except ''A. baylyi'' ADP1''ΔmutS''. ECNR2 plate was sparse, but had colonies. Al...") (top)
- 23:16, 3 October 2012 (diff | hist) N User:DrJones1935/10 July 2012 (Created page with "====I. Streak cells for upcoming transformation with pBAV1K-T5-gfp plasmid==== *Using sterilized inoculating loop, scrape top layer of ice of glycerol stock and streak cells ont...") (top)
- 23:15, 3 October 2012 (diff | hist) N User:DrJones1935/5 July 2012 (Created page with "====I. Ordered Cross-over PCR Primers from Keck==== *Primers were ordered with the name/sequence in the following table: {| class="wikitable" |- ! Name !! Tm (Tm overhang/Tm an...") (top)
- 23:14, 3 October 2012 (diff | hist) N User:DrJones1935/30 June 2012 (Created page with "====I. Check overnight cultures for growth==== *As of ~2:30 PM when I got in, both cultures were cloudy corresponding to saturation ====II. Make 60% glycerol stock from 100% st...") (top)
- 23:14, 3 October 2012 (diff | hist) N User:DrJones1935/29 June 2012 (Created page with "====I. Check culture and plate for overnight growth==== *''Results: No growth in the ECNR2 culture at 37 <sup>o</sup>C, confirming that these cultures will not grow at these tem...") (top)
- 23:13, 3 October 2012 (diff | hist) N User:DrJones1935/28 June 2012 (Created page with "====I: Inoculate ECNR2 Cultures from Natalie Ma's Plate(from 7 Jun 2012)==== *Inoculate one isolated colony into ~2 mL of LB media in sterile culture tube (x2) *P...") (top)
- 23:12, 3 October 2012 (diff | hist) Nm User:DrJones1935/27 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (3 total) from Successful Overnight Culture==== *Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3) :*SIMD43 *Add 200 uL of...") (top)
- 23:12, 3 October 2012 (diff | hist) N User:DrJones1935/26 June 2012 (Created page with "====I. Inoculate Liquid Cultures for Cultures Which Failed to Grow==== *Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/25 June 2012 (Created page with "====I. Inoculate Liquid Cultures for Cultures Which Failed to Grow==== *Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/22 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (15 total) from Successful Overnight Cultures==== *Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3 per culture) :*SIMD40 ...") (top)
- 23:11, 3 October 2012 (diff | hist) N User:DrJones1935/21 June 2012 (Created page with "====I: Make 15% Glycerol Stocks (42 total) from Successful Overnight Cultures==== *Chill an aliquot of sterile 60% glycerol on ice *Transfer 600 uL of each liquid culture to eac...") (top)
- 23:03, 3 October 2012 (diff | hist) N User:DrJones1935/20 June 2012 (Created page with "====I. Make 100 mg/ mL <span style="color:#FF0000">Ampicillin</span> Stock Solution (small) for Plates/Liquid Cultures==== *Weigh out ''(150 mg)'' 150.32 mg of <span style="colo...") (top)
- 23:02, 3 October 2012 (diff | hist) N User:DrJones1935/15 June 2012 (Created page with "''Note: Professor Isaacs brought samples of'' B. subtilis ''1A40 from the Breaker Lab. There was 1 plate and 1 liquid culture'' ====I: Make 15% Glycerol Stocks (4) from ''B. sub...") (top)
- 23:02, 3 October 2012 (diff | hist) N User:DrJones1935/12 July 2012 (Created page with "====I: Check WT B. subtilis Liquid Culture for Growth==== *RESULTS: Culture appears uniformly cloudy consistent with overnight growth. The glycerol stocks are viable.")
- 23:01, 3 October 2012 (diff | hist) N User:DrJones1935/11 June 2012 (Created page with "====I. Inoculate WT ''B. subtilis'' Liquid Culture to Test Glycerol Stocks==== *Add 5 mL of RT Nutrient Broth to a clean 50 mL conical tube *Remove 1 WT ''B. subtilis'' glycerol...") (top)
- 23:00, 3 October 2012 (diff | hist) N User:DrJones1935/8 June 2012 (Created page with "====I. Measure OD600 of WT ''B. subtilis'' Grown Overnight with 10% Dilution==== *Remove 100 uL of saturated culture from 125 mL E-flask *Mix with 900 uL of plain Nutrient Broth...") (top)
- 23:00, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Spencer Katz)
- 22:59, 3 October 2012 (diff | hist) User:DrJones1935/7 June 2012 (top)
- 22:59, 3 October 2012 (diff | hist) N User:DrJones1935/7 June 2012 (Created page with "thumb == Laboratory Notebook for Spencer Katz == ---- ===Lab Notes, Thurs, 7 Jun 2012=== ---- ====I. Inoculate Wild Type (WT) '...")
- 22:58, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Notebook entries, by date)
- 22:52, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Notebook entries, by date)
- 22:49, 3 October 2012 (diff | hist) Team:Yale/Notebook (→Notebook entries, by date)
- 22:42, 3 October 2012 (diff | hist) Team:Yale/Parts (top)
- 22:41, 3 October 2012 (diff | hist) Team:Yale/Parts
- 22:40, 3 October 2012 (diff | hist) Team:Yale/Parts
- 22:40, 3 October 2012 (diff | hist) Team:Yale/Modeling
- 22:38, 3 October 2012 (diff | hist) Team:Yale/Modeling
- 22:35, 3 October 2012 (diff | hist) Team:Yale/Modeling
- 22:34, 3 October 2012 (diff | hist) Team:Yale/Modeling
- 22:34, 3 October 2012 (diff | hist) Team:Yale/Modeling
- 22:26, 3 October 2012 (diff | hist) Team:Yale/Safety (→Safety Questions:)
- 22:26, 3 October 2012 (diff | hist) Team:Yale/Attributions (→Attributions for work done)
- 22:25, 3 October 2012 (diff | hist) Team:Yale/Attributions
- 22:25, 3 October 2012 (diff | hist) Team:Yale/Attributions
- 22:22, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:21, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:20, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:19, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:19, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:18, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:17, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:17, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:15, 3 October 2012 (diff | hist) Team:Yale/Notebook
- 22:14, 3 October 2012 (diff | hist) Team:Yale/Notebook
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