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From 2012.igem.org

I. Check culture and plate for overnight growth

• Results: No growth in the ECNR2 culture at 37 ^oC, confirming that these cultures will not grow at these temperatures. Modest colony growth in the two strains on the plate from the ~34 - 35 ^oC static incubator, moreso in ECNR2 than ECNR1. There are enough isolated colonies of both strains for liquid cultures.

II. Lower Incubator Temperature

• The shaking incubator now has an internal temperature of 33 ^oC consistently.

III. Inoculate ECNR1 and ECNR2 Cultures from Plate streaked by Prof. Isaacs

• Inoculate one isolated colony into ~2 mL of LB media in a sterile culture tube for each strain (2 tubes total, ~2 PM)
• Return plate to static incubator
• Place tubes in 33 ^oC incubator and check on cultures after 4 hours to see if they have grown at all

No visible turbidity at 4 hours, leaving it overnight. ~ Srk3 (talk) 18:01, 29 June 2012 (EDT)