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From 2012.igem.org

I. Make 100 mg/ mL Ampicillin Stock Solution (small) for Plates/Liquid Cultures

• Weigh out (150 mg) 150.32 mg of Ampicillin powder from refrigerator
• Vortex with 1.0 mL of dH₂O in microcentrifuge tube
• Bring to 1.5 mL with dH₂O, mix gently by pipetting
• Filter sterilize into a clean microcentrifuge tube
• Working concentration = 100 ug/mL

II. Inoculate Liquid Cultures for Cultures Received Today

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• Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the following table in sterile culture tubes

Strain	Media
E. coli ECNR1	LB
E. coli ECNR2	LB
E. coli MG1655	LB
A. baylyi ADP1	LB
E. coli SIMD40	LB
E. coli SIMD41	LB
E. coli SIMD42	LB
E. coli SIMD43	LB
E. coli SIMD44	LB
E. coli SIMD45	LB
E. coli SIMD46	LB
E. coli SIMD47	LB
E. coli SIMD48	LB
E. coli SIMD49	LB
E. coli SIMD50	LB
E. coli SIMD57	LB + Amp (100 ug/mL)'
E. coli SIMD58	LB + Amp (100 ug/mL)
E. coli SIMD59	LB + Amp (100 ug/mL)
E. coli SIMD60	LB + Amp (100 ug/mL)
E. coli SIMD61	LB + Amp (100 ug/mL)

• 'For LB+Amp, 14 uL of Amp stock (see above) was added to 14 mL of LB and mixed thoroughly before dispensing

• Incubate cultures overnight with shaking at 37 ^oC