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From 2012.igem.org

I: Make 15% Glycerol Stocks (3 total) from Successful Overnight Culture

• Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3)

• SIMD43

• Add 200 uL of 60% glycerol to each tube (x3)
• Vortex to mix thoroughly
• Store tubes in the -80 ^oC freezer
• Discard any remaining liquid culture from above cell line

Note: ECNR1 strain still has not grown. I am leaving culture in incubator to see if it is just slow like ECNR2 ~ Srk3 11:34, 27 June 2012 (EDT)

Note: Professor Isaacs said that both of the ECNR strains should be robust, so we will scrap the ECNR cultures and streak out new plates. ~ Srk3 12:29, 28 June 2012 (EDT)