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From 2012.igem.org

I. Inoculate Liquid Cultures for Cultures Which Failed to Grow

• Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the following table in sterile culture tubes

Strain	Media
E. coli ECNR1	LB
E. coli SIMD43	LB

• Incubate cultures overnight with shaking at 37 ^oC