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From 2012.igem.org

I: Make 15% Glycerol Stocks (15 total) from Successful Overnight Cultures

• Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3 per culture)

• SIMD40
• SIMD42
• SIMD45
• SIMD50
• SIMD57

• Add 200 uL of 60% glycerol to each tube (x3 per culture)
• Vortex to mix thoroughly
• Store tubes in the -80 ^oC freezer
• Discard any remaining liquid culture from above cell lines

Note: The ECNR1 and SIMD43 strains have still not grown. Liquid cultures will be left over the weekend.