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Team:Groningen/Notebook/Wetwork 16August2012
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Revision as of 12:27, 30 August 2012
Tom
Ligation of Psb1c3 with Fnr promotor combined to GFP. For the digestion of Psb1c3, 258.2 ng was used. For the digestion of Fnr promotor and GFP, 239 ng was used. Ligation was done with 25.82 ng Psb1c3 and 36 ng of Fnr promotor + GFP.
Result: After transformation and plasmid isolation all the colonies contained empty plasmids.
