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Team:Grenoble/Biology/Protocols/Glycerol
From 2012.igem.org
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You need to: | You need to: | ||
<ol> | <ol> | ||
| - | <li>Grow a liquid culture overnight</li> | + | <li>Grow a liquid culture overnight.</li> |
| - | <li>Prepare sterile 80% glycerol</li> | + | <li>Prepare sterile 80% glycerol.</li> |
| - | <li>Mix 200 µL of glycerol and 800 µL of liquid culture</li> | + | <li>Mix 200 µL of glycerol and 800 µL of liquid culture.</li> |
</ol> | </ol> | ||
<br/> | <br/> | ||
Revision as of 19:03, 27 August 2012
Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- Grow a liquid culture overnight.
- Prepare sterile 80% glycerol.
- Mix 200 µL of glycerol and 800 µL of liquid culture.
Then store the glycerol stock at -80°C.
