Team:Grenoble/Biology/Protocols/Glycerol

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Glycerol stock preparation

Goal

Prepare glycerol stock is a way to store bacteria during a long period.

Protocol

You need to:
  1. grow a liquid culture overnight
  2. prepare sterile 80% glycerol
  3. mix 200 L of glycerol and 800 L of liquid culture
Then store the glycerol stock at -80°C.

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