Team:Grenoble/Biology/Protocols

From 2012.igem.org

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     <h2>Molecular biological techniques</h2>
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    <ul>
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        <li>PCR using a GoTaq polymerase</li>
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        <li>PCR using a HF Phusion polymerase</li>
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        <li>Gibson Assembly</li>
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        <li>Restriction enzyme digest</li>
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        <li>Ligation</li>
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        <li>Miniprep</li>
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    </ul>
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    <h2>Gel migration</h2>
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    <ul>
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        <li>Gel electrophoresis</li>
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        <li>DNA extraction</li>
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    </ul>
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    <h2>Test techniques</h2>
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    <ul>
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        <li>"AND" gate test</li>
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    </ul>
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Revision as of 13:16, 22 August 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

Protocols

Here you can find the protocols used by the team. There are

Cell culture

  • Medium compositions
  • LB Agar plates preparation
  • Glycerol stock production

BioBricks utilisation

  • BioBricks extraction
  • Chemically competent cells production
  • Transformation of E. coli competent cells (protocol 1)
  • Transformation of E. coli competent cells (protocol 2)

Molecular biological techniques

  • PCR using a GoTaq polymerase
  • PCR using a HF Phusion polymerase
  • Gibson Assembly
  • Restriction enzyme digest
  • Ligation
  • Miniprep

Gel migration

  • Gel electrophoresis
  • DNA extraction

Test techniques

  • "AND" gate test

Retrieved from "http://2012.igem.org/Team:Grenoble/Biology/Protocols"