Team:Grenoble/Biology/Notebook/July/week 32
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<center><img src="https://static.igem.org/mediawiki/2012/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center> | ||
- | <div class="legend"><p><u>Migration result for a 1.8% TAE agarose gel</u><br/> | + | <div class="legend"><p><center><u>Migration result for a 1.8% TAE agarose gel</u><br/> |
- | <i>(the DNA ladder scale is in kb)</i></p> | + | <i>(the DNA ladder scale is in kb)</i></center></p> |
<ul><li><b>Lane 1:</b> pLAC_rsmY (pSB1A3) <b>3</b> miniprep product</li> | <ul><li><b>Lane 1:</b> pLAC_rsmY (pSB1A3) <b>3</b> miniprep product</li> | ||
<li><b>Lane 2:</b> pLAC_rsmY (pSB1A3) <b>2</b> miniprep product</li> | <li><b>Lane 2:</b> pLAC_rsmY (pSB1A3) <b>2</b> miniprep product</li> |
Revision as of 14:26, 20 August 2012
August
Week 31 • Week 32 • Week 33 • Week 34 • Week 35Week 32: August 06th to 12th
Goal of the week:
Monday, August 06th:
We wanted to check if the Gibson Assemblies (12/08/01) worked well.To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
(the DNA ladder scale is in kb)
- Lane 1: pLAC_rsmY (pSB1A3) 3 miniprep product
- Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
- Lane 3: pLAC_rsmY (pSB1A3) 1 miniprep product
- Lane 4: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
- Lane 5: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
- Lane 6: DNA ladder 1kb (biolabs)
- Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
- Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
- Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
- Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
- Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
- Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
- Lane 13: DNA ladder 1kb (biolabs)
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.
To separate the digestion products, we prepared two 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
(the DNA ladder scale is in kb)
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)