Team:Grenoble/Biology/Notebook/June/week 25

From 2012.igem.org

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<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_25">Week 25</a> •  
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_25">Week 25</a> •  
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_26">Week 26</a>
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_26">Week 26</a>
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<h1> Week 25: June 18<span class="exposant">th</span> to 24<span class="exposant">th</span> </h1>
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After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature: <br/>
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<center><img src="https://static.igem.org/mediawiki/2012/d/d5/Network_cAMP-CRP.jpg" alt="pLAC_rsmY"/></center>
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We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.<br/>
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<h1> Week 24: June 11<span class="exposant">th</span> to 17<span class="exposant">th</span> </h1>
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During this week, we worked on the amplifier design (RsmA-rsmY system): <br/>
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<center><img src="https://static.igem.org/mediawiki/2012/3/32/RsmA-rsmY.jpg" alt="amplifier_1"/></center><br/>
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We looked for the plasmids on which we wanted to insert the selected biobricks in order to construct the amplifiers and to test them. <br/>
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We also sat up the working hypothesis in order to test the RsmA-rsmY system. We first decided to test the RBS fha efficiency, the RsmA inhibition ability and the rsmY ability to remove this inhibition.<br/>
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In order to do this, we looked for the biobricks involved in this genetic network and for the sequences of RsmA, rsmY and fha.<br/>
 
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We compared the fha and Csra (<i>E. coli</i> fha equivalent) sequences found in the literature, with the one used by the iGEM Grenoble 2011 team and we realised that the sequence was too long. We thus decided to take a shorter sequence (47bp).<br/>
 
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We searched in the literature to find a way to build a detection module.
We searched in the literature to find a way to build a detection module.
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Latest revision as of 13:50, 20 August 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

June

Week 23 • Week 24 • Week 25 • Week 26

Week 25: June 18th to 24th

After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature:

pLAC_rsmY

We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.

We looked for the plasmids on which we wanted to insert the selected biobricks in order to construct the amplifiers and to test them.

We searched in the literature to find a way to build a detection module.

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