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Revision as of 17:57, 2 August 2012 (view source) RenskevR (Talk | contribs) (Created page with "{{HeaderGroningen2012}} Arjan Did last TAMC measurement (24:00 sample).") ← Older edit		Revision as of 16:26, 7 August 2012 (view source) Nisa.purwanto (Talk | contribs) Newer edit →
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	Did last TAMC measurement (24:00 sample).		Did last TAMC measurement (24:00 sample).
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		+	Nisa
		+
		+	Re-did E. coli DH5a transformation (as performed on Sunday)
		+
		+	Emeraldo
		+
		+	Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI
		+
		+	Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2
		+
		+	Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis.

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Revision as of 16:26, 7 August 2012

Arjan

Did last TAMC measurement (24:00 sample).

Nisa

Re-did E. coli DH5a transformation (as performed on Sunday)

Emeraldo

Digestion of lycopene (BBa_K274110) and (expected) B. subtilis backbone pSac-Cm using EcoRI and SpeI

Ligated the two fragments together (at 25oC, 2 h). Ligation ratio backbone:insert gene = 1:2

Introduced the ligated fragment into E. coli DH5a and B. subtilis. Ampicillin was used for selection in E. coli and chloramphenicol was used for selection in B. subtilis.