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Team:Groningen/Notebook/Wetwork 16July2012
From 2012.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
Nisa | Nisa | ||
| - | PCR of GFP with the recieved primers | + | PCR of GFP (optimized for B. subtilis) with the recieved primers |
T melting Fwd= 57C | T melting Fwd= 57C | ||
T melting Rev = 61C | T melting Rev = 61C | ||
| - | Therefore, make a gradient from | + | Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65) |
Ligation primer | Ligation primer | ||
| - | idea: ligate mulitple fragments of DNA without T4 ligase, but using PCR | + | idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method. |
Revision as of 09:13, 24 July 2012
Nisa
PCR of GFP (optimized for B. subtilis) with the recieved primers T melting Fwd= 57C T melting Rev = 61C Therefore, make a gradient from 55C-65C (55, 57, 59,61,63,65)
Ligation primer
idea: ligate mulitple fragments of DNA without T4 ligase, but using the PCR method.
